Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting
Identifieur interne : 000712 ( Main/Exploration ); précédent : 000711; suivant : 000713Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting
Auteurs : Marcos Quintela-Baluja [Espagne] ; Karola Böhme [Espagne] ; Inmaculada C. Fernández-No [Espagne] ; Stefano Morandi [Italie] ; Mohammed E. Alnakip [Espagne] ; Sonia Caama O-Antelo [Espagne] ; Jorge Barros-Velázquez [Espagne] ; Pilar Calo-Mata [Espagne]Source :
- ELECTROPHORESIS [ 0173-0835 ] ; 2013-08.
Abstract
Enterococcus is a controversial genus due to its great variability; this genus includes pathogenic strains, spoilage strains, and apparently safe strains including some probiotic strains. Previous studies focused on the characterization of strains of Enterococcus spp. involved in nosocomial infections. However, little research has been conducted on Enterococcus strains in foodstuffs. In the present work, 36 strains of different species of Enterococcus have been characterized by means of MALDI‐TOF MS, resulting in highly specific mass spectral fingerprints. Characteristic peak masses common to certain bacterial species of Enterococcus have been identified. Thus, a peak at m/z 4426 ± 1 was assigned as a genus‐specific biomarker. In addition, phyloproteomic relationships based on the mass spectral data were compared to the results of a phylogenetic analysis based on the 16S rRNA gene sequence. A better grouping at the species level was observed in the phyloproteomic tree, especially for the Enterococcus faecium group. Presumably, the assortment of some strains or ecotypes could be related to their ecological niche specialization. The approach described in this study leads the way toward the rapid and specific identification of different strains and species of Enterococcus in food based on molecular protein markers, aiming at the early detection of pathogenic strains and strains implicated in food poisoning or food spoilage.
Url:
DOI: 10.1002/elps.201200699
Affiliations:
- Espagne, Italie
- Galice, Lombardie
- Milan, Santiago de Compostela
- Université de Saint-Jacques-de-Compostelle
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000737
- to stream Istex, to step Curation: 000737
- to stream Istex, to step Checkpoint: 000369
- to stream Main, to step Merge: 000713
- to stream Main, to step Curation: 000712
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting</title><author><name sortKey="Quintela Aluja, Marcos" sort="Quintela Aluja, Marcos" uniqKey="Quintela Aluja M" first="Marcos" last="Quintela-Baluja">Marcos Quintela-Baluja</name></author><author><name sortKey="Bohme, Karola" sort="Bohme, Karola" uniqKey="Bohme K" first="Karola" last="Böhme">Karola Böhme</name></author><author><name sortKey="Fernandez O, Inmaculada C" sort="Fernandez O, Inmaculada C" uniqKey="Fernandez O I" first="Inmaculada C." last="Fernández-No">Inmaculada C. Fernández-No</name></author><author><name sortKey="Morandi, Stefano" sort="Morandi, Stefano" uniqKey="Morandi S" first="Stefano" last="Morandi">Stefano Morandi</name></author><author><name sortKey="Alnakip, Mohammed E" sort="Alnakip, Mohammed E" uniqKey="Alnakip M" first="Mohammed E." last="Alnakip">Mohammed E. Alnakip</name></author><author><name sortKey="Caama O Ntelo, Sonia" sort="Caama O Ntelo, Sonia" uniqKey="Caama O Ntelo S" first="Sonia" last="Caama O-Antelo">Sonia Caama O-Antelo</name></author><author><name sortKey="Barros Elazquez, Jorge" sort="Barros Elazquez, Jorge" uniqKey="Barros Elazquez J" first="Jorge" last="Barros-Velázquez">Jorge Barros-Velázquez</name></author><author><name sortKey="Calo Ata, Pilar" sort="Calo Ata, Pilar" uniqKey="Calo Ata P" first="Pilar" last="Calo-Mata">Pilar Calo-Mata</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CAF6CCF5290773DBD68394F37F11A3E28E043DAF</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1002/elps.201200699</idno><idno type="url">https://api.istex.fr/document/CAF6CCF5290773DBD68394F37F11A3E28E043DAF/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000737</idno><idno type="wicri:Area/Istex/Curation">000737</idno><idno type="wicri:Area/Istex/Checkpoint">000369</idno><idno type="wicri:doubleKey">0173-0835:2013:Quintela Aluja M:characterization:of:different</idno><idno type="wicri:Area/Main/Merge">000713</idno><idno type="wicri:Area/Main/Curation">000712</idno><idno type="wicri:Area/Main/Exploration">000712</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting</title><author><name sortKey="Quintela Aluja, Marcos" sort="Quintela Aluja, Marcos" uniqKey="Quintela Aluja M" first="Marcos" last="Quintela-Baluja">Marcos Quintela-Baluja</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Bohme, Karola" sort="Bohme, Karola" uniqKey="Bohme K" first="Karola" last="Böhme">Karola Böhme</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Fernandez O, Inmaculada C" sort="Fernandez O, Inmaculada C" uniqKey="Fernandez O I" first="Inmaculada C." last="Fernández-No">Inmaculada C. Fernández-No</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Morandi, Stefano" sort="Morandi, Stefano" uniqKey="Morandi S" first="Stefano" last="Morandi">Stefano Morandi</name><affiliation wicri:level="3"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Sciences of Food Production (ISPA–CNR), Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Alnakip, Mohammed E" sort="Alnakip, Mohammed E" uniqKey="Alnakip M" first="Mohammed E." last="Alnakip">Mohammed E. Alnakip</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Caama O Ntelo, Sonia" sort="Caama O Ntelo, Sonia" uniqKey="Caama O Ntelo S" first="Sonia" last="Caama O-Antelo">Sonia Caama O-Antelo</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Barros Elazquez, Jorge" sort="Barros Elazquez, Jorge" uniqKey="Barros Elazquez J" first="Jorge" last="Barros-Velázquez">Jorge Barros-Velázquez</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation></author><author><name sortKey="Calo Ata, Pilar" sort="Calo Ata, Pilar" uniqKey="Calo Ata P" first="Pilar" last="Calo-Mata">Pilar Calo-Mata</name><affiliation wicri:level="4"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo</wicri:regionArea><orgName type="university">Université de Saint-Jacques-de-Compostelle</orgName><placeName><settlement type="city">Santiago de Compostela</settlement><region nuts="2" type="region">Galice</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Espagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j">ELECTROPHORESIS</title><title level="j" type="abbrev">ELECTROPHORESIS</title><idno type="ISSN">0173-0835</idno><idno type="eISSN">1522-2683</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-08">2013-08</date><biblScope unit="volume">34</biblScope><biblScope unit="issue">15</biblScope><biblScope unit="page" from="2240">2240</biblScope><biblScope unit="page" to="2250">2250</biblScope></imprint><idno type="ISSN">0173-0835</idno></series><idno type="istex">CAF6CCF5290773DBD68394F37F11A3E28E043DAF</idno><idno type="DOI">10.1002/elps.201200699</idno><idno type="ArticleID">ELPS4761</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0173-0835</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Enterococcus is a controversial genus due to its great variability; this genus includes pathogenic strains, spoilage strains, and apparently safe strains including some probiotic strains. Previous studies focused on the characterization of strains of Enterococcus spp. involved in nosocomial infections. However, little research has been conducted on Enterococcus strains in foodstuffs. In the present work, 36 strains of different species of Enterococcus have been characterized by means of MALDI‐TOF MS, resulting in highly specific mass spectral fingerprints. Characteristic peak masses common to certain bacterial species of Enterococcus have been identified. Thus, a peak at m/z 4426 ± 1 was assigned as a genus‐specific biomarker. In addition, phyloproteomic relationships based on the mass spectral data were compared to the results of a phylogenetic analysis based on the 16S rRNA gene sequence. A better grouping at the species level was observed in the phyloproteomic tree, especially for the Enterococcus faecium group. Presumably, the assortment of some strains or ecotypes could be related to their ecological niche specialization. The approach described in this study leads the way toward the rapid and specific identification of different strains and species of Enterococcus in food based on molecular protein markers, aiming at the early detection of pathogenic strains and strains implicated in food poisoning or food spoilage.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>Italie</li></country><region><li>Galice</li><li>Lombardie</li></region><settlement><li>Milan</li><li>Santiago de Compostela</li></settlement><orgName><li>Université de Saint-Jacques-de-Compostelle</li></orgName></list><tree><country name="Espagne"><region name="Galice"><name sortKey="Quintela Aluja, Marcos" sort="Quintela Aluja, Marcos" uniqKey="Quintela Aluja M" first="Marcos" last="Quintela-Baluja">Marcos Quintela-Baluja</name></region><name sortKey="Alnakip, Mohammed E" sort="Alnakip, Mohammed E" uniqKey="Alnakip M" first="Mohammed E." last="Alnakip">Mohammed E. Alnakip</name><name sortKey="Barros Elazquez, Jorge" sort="Barros Elazquez, Jorge" uniqKey="Barros Elazquez J" first="Jorge" last="Barros-Velázquez">Jorge Barros-Velázquez</name><name sortKey="Bohme, Karola" sort="Bohme, Karola" uniqKey="Bohme K" first="Karola" last="Böhme">Karola Böhme</name><name sortKey="Caama O Ntelo, Sonia" sort="Caama O Ntelo, Sonia" uniqKey="Caama O Ntelo S" first="Sonia" last="Caama O-Antelo">Sonia Caama O-Antelo</name><name sortKey="Calo Ata, Pilar" sort="Calo Ata, Pilar" uniqKey="Calo Ata P" first="Pilar" last="Calo-Mata">Pilar Calo-Mata</name><name sortKey="Calo Ata, Pilar" sort="Calo Ata, Pilar" uniqKey="Calo Ata P" first="Pilar" last="Calo-Mata">Pilar Calo-Mata</name><name sortKey="Fernandez O, Inmaculada C" sort="Fernandez O, Inmaculada C" uniqKey="Fernandez O I" first="Inmaculada C." last="Fernández-No">Inmaculada C. Fernández-No</name></country><country name="Italie"><region name="Lombardie"><name sortKey="Morandi, Stefano" sort="Morandi, Stefano" uniqKey="Morandi S" first="Stefano" last="Morandi">Stefano Morandi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000712 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000712 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CAF6CCF5290773DBD68394F37F11A3E28E043DAF
|texte= Characterization of different food‐isolated Enterococcus strains by MALDI‐TOF mass fingerprinting
}}
| This area was generated with Dilib version V0.6.25. |  |